abstract：:We have investigated RNA recombination among poliovirus genomes by analyzing both intratypic and intertypic recombinant crosses involving the same defined genetic markers. Sequence analysis of the recombinant junctions of 13 nonsibling intertypic recombinants showed that intertypic RNA recombination is not site-specif...

journal_title：Cell

authors： Kirkegaard K,Baltimore D

更新日期：1986-11-07 00:00:00

abstract：:Forms of RNA polymerase I prepared from growing or encysted Acanthamoeba are equal in the ability to transcribe poly(dl:dC). Polymerase from cysts, whose rRNA genes are transcriptionally inactive, is unable to utilize the rDNA promoter in vitro, whereas the transcription initiation factor from cysts is fully able to b...

journal_title：Cell

authors： Bateman E,Paule MR

更新日期：1986-11-07 00:00:00

abstract：:Here we characterize the T-cell-activating protein (TAP), an Ly-6 gene product involved in T cell activation, as a glycoprotein with a molecular weight of 10-12 kd under nonreducing conditions and 15-18 kd under reducing ones. Two of the three bands that are precipitated from metabolically labeled cells are expressed ...

journal_title：Cell

authors： Reiser H,Oettgen H,Yeh ET,Terhorst C,Low MG,Benacerraf B,Rock KL

更新日期：1986-11-07 00:00:00

abstract：:Cytotoxic T lymphocytes and lymphocytes with NK-like activity contain a serine esterase activity which has been localized to their cytoplasmic granules by cytochemistry and subcellular fractionation studies. The serine esterase-specific inhibitor 3H-DFP labels two protein species in the granules. The two proteins, ref...

journal_title：Cell

authors： Young JD,Leong LG,Liu CC,Damiano A,Wall DA,Cohn ZA

更新日期：1986-10-24 00:00:00

abstract：:A cDNA clone encoding a novel hematopoietic growth factor activity produced by a gibbon T cell line has been identified using a mammalian cell expression cloning system. The sequence of this cDNA proved to have significant homology to the sequence encoding murine interleukin 3 (IL-3). The human gene, which was readily...

journal_title：Cell

authors： Yang YC,Ciarletta AB,Temple PA,Chung MP,Kovacic S,Witek-Giannotti JS,Leary AC,Kriz R,Donahue RE,Wong GG

更新日期：1986-10-10 00:00:00

abstract：:The human invariant chain (I gamma) of class II histocompatibility antigens spans the membrane of the endoplasmic reticulum once. It exposes a small amino-terminal domain on the cytoplasmic side and a carboxy-terminal, glycosylated domain on the exoplasmic side of the membrane. When the exoplasmic domain of I gamma is...

journal_title：Cell

authors： Lipp J,Dobberstein B

更新日期：1986-09-26 00:00:00

abstract：:cDNA clones of the Drosophila epidermal growth factor receptor homolog (DER) gene were isolated and sequenced. The deduced amino acid sequence shows a similar degree of homology to the human epidermal growth factor receptor and to the rat and human neu proteins; the most striking difference is the addition of a third ...

journal_title：Cell

authors： Schejter ED,Segal D,Glazer L,Shilo BZ

更新日期：1986-09-26 00:00:00

abstract：:The adenovirus E1a region encodes two closely related gene products: 243 and 289 amino acid phosphoproteins. These proteins differ in their primary sequence only by 46 amino acids unique to the 289 amino acid protein. By constructing single-base substitution mutants we localized two functional regions of these E1a pro...

journal_title：Cell

authors： Lillie JW,Green M,Green MR

更新日期：1986-09-26 00:00:00

abstract：:Lambda integrative recombination depends on supercoiling of the phage attachment site, attP. Using dimethylsulfate protection and indirect end-labeling, the interaction of the recombination proteins Int and IHF with supercoiled and linear attP has been studied. Supercoiling enhances the binding of Int to attP, but not...

journal_title：Cell

authors： Richet E,Abcarian P,Nash HA

更新日期：1986-09-26 00:00:00

abstract：:The hemagglutinin of influenza virus is synthesized as a monomeric subunit that is cotranslationally translocated across the membrane of the rough endoplasmic reticulum. We show that folding and assembly of hemagglutinin monomers into trimeric structures takes approximately 7-10 min and is completed before the protein...

journal_title：Cell

authors： Gething MJ,McCammon K,Sambrook J

更新日期：1986-09-12 00:00:00

abstract：:Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5' and 3' splice sites i...

journal_title：Cell

authors： Reed R,Maniatis T

更新日期：1986-08-29 00:00:00

abstract：:We have shown previously that the putative oncogene pim-1 is frequently activated by provirus insertion in murine leukemia virus-induced T cell lymphomas. Here we describe the structure of the pim-1 gene as determined by sequencing genomic and cDNA clones. The gene has an open reading frame, encoding a protein of 313 ...

journal_title：Cell

authors： Selten G,Cuypers HT,Boelens W,Robanus-Maandag E,Verbeek J,Domen J,van Beveren C,Berns A

更新日期：1986-08-15 00:00:00

abstract：:Mouse monoclonal antibody 4-I-244 detects a developmentally regulated antigen in embryos of Volvox carteri and inhibits specifically the morphogenetic process of inversion (the process by which the embryo turns inside out). Antigen 4-I-244 was chemically characterized as a complex phytosphingolipid containing the neut...

journal_title：Cell

authors： Wenzl S,Sumper M

更新日期：1986-08-15 00:00:00

abstract：:Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellular transformation ...

journal_title：Cell

authors： Rosenthal A,Lindquist PB,Bringman TS,Goeddel DV,Derynck R

更新日期：1986-07-18 00:00:00

abstract：:In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis beta 1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, wh...

journal_title：Cell

authors： Mason PJ,Elkington JA,Lloyd MM,Jones MB,Williams JG

更新日期：1986-07-18 00:00:00

abstract：:Different hematopoietic cells produce minute amounts of beta-related interferon (IFN) following induction of differentiation by chemical or natural inducers. The endogenous IFN binds to type I cell surface receptors and modulates gene expression in the producer cells. We show that self-induction of two members of the ...

journal_title：Cell

authors： Resnitzky D,Yarden A,Zipori D,Kimchi A

更新日期：1986-07-04 00:00:00

abstract：:A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains ...

journal_title：Cell

authors： McKnight GL,O'Hara PJ,Parker ML

更新日期：1986-07-04 00:00:00

abstract：:Forskolin- and cholera toxin-activated adenylate cyclase activity increases between the morula and blastocyst stages of mouse preimplantation development, as assessed by the ability of these agents to increase embryonic cAMP levels. Development of activatable adenylate cyclase requires transcription but is independent...

journal_title：Cell

authors： Manejwala F,Kaji E,Schultz RM

更新日期：1986-07-04 00:00:00

abstract：:We present genetic evidence that the tetracycline resistance element Tn10 transposes by a nonreplicative mechanism. Heteroduplex Tn10 elements containing three single base pair mismatches were constructed on lambda phage genomes and allowed to transpose from lambda into the bacterial chromosome. Analysis of TetR colon...

journal_title：Cell

authors： Bender J,Kleckner N

更新日期：1986-06-20 00:00:00

abstract：:Nuclear and cytoplasmic poly(A)-binding proteins have been purified from Saccharomyces cerevisiae, and antisera have been used to isolate a gene that encodes them. The gene occurs in a single copy on chromosome 5 and gives rise to a unique, unspliced 2.1 kb transcript. The nuclear protein appears to be derived from th...

journal_title：Cell

authors： Sachs AB,Bond MW,Kornberg RD

更新日期：1986-06-20 00:00:00

abstract：:We have isolated the bovine and human genes for Müllerian inhibiting substance (MIS), a testicular glycoprotein that causes regression of the Müllerian duct during development of the male embryo. The mRNA sequence of bovine MIS, determined from an analysis of cDNA and genomic clones, codes for a protein of 575 amino a...

journal_title：Cell

authors： Cate RL,Mattaliano RJ,Hession C,Tizard R,Farber NM,Cheung A,Ninfa EG,Frey AZ,Gash DJ,Chow EP

更新日期：1986-06-06 00:00:00

abstract：:To determine the extent and nature of genetic variation present in independent isolates of HTLV-III/LAV, the nucleotide sequences of the entire envelope gene and parts of gag and pol were determined for two AIDS viruses. The results indicated that variation throughout the viral genome is extensive and that the envelop...

journal_title：Cell

authors： Starcich BR,Hahn BH,Shaw GM,McNeely PD,Modrow S,Wolf H,Parks ES,Parks WP,Josephs SF,Gallo RC

更新日期：1986-06-06 00:00:00

abstract：:The genetic structure of Mason-Pfizer monkey virus (MPMV), a D-type retrovirus, has been determined. In addition to the viral gag, pol, and env genes is an ORF overlapping both gag and pol and that encodes the viral protease. Surprisingly, the MPMV env protein is highly homologous to that of the avian C-type virus, re...

journal_title：Cell

authors： Sonigo P,Barker C,Hunter E,Wain-Hobson S

更新日期：1986-05-09 00:00:00

abstract：:Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the ...

journal_title：Cell

authors： Kinutani M,Coltey M,Le Douarin NM

更新日期：1986-04-25 00:00:00

abstract：:A complete human gamma 1 heavy chain gene (HIG1) was transferred into mouse cells by protoplast fusion. The HIG1 gene was strongly expressed in mouse myeloma cells but not in mouse fibroblasts (L cells). Nuclear extracts from myeloma cells were injected into L cell transformants containing one copy of the HIG1 gene; t...

journal_title：Cell

authors： Maeda H,Kitamura D,Kudo A,Araki K,Watanabe T

更新日期：1986-04-11 00:00:00

abstract：:Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now p...

journal_title：Cell

authors： Huang CC,Hay N,Bishop JM

更新日期：1986-03-28 00:00:00

abstract：:By chromosome walking in the major histocompatibility complex (MHC) of the BALB/c mouse, we have linked the K region to the I region at the molecular level. Forty-nine overlapping cosmid clones define a stretch of about 600 kb of DNA containing 2 class I and 7 class II genes. Eleven independent recombination events we...

journal_title：Cell

authors： Steinmetz M,Stephan D,Fischer Lindahl K

更新日期：1986-03-28 00:00:00

abstract：:To test the functional consequence of phosphorylation of the EGF receptor at Thr 654 by protein kinase C, the normal Thr 654 human EGF receptor cDNA or a mutant encoding an Ala 654 were expressed in heterologous cells. In cell lines expressing both the Thr 654 and Ala 654 receptors, functional cell-surface Thr 654 rec...

journal_title：Cell

authors： Lin CR,Chen WS,Lazar CS,Carpenter CD,Gill GN,Evans RM,Rosenfeld MG

更新日期：1986-03-28 00:00:00

abstract：:The mouse t haplotypes show defects in spermatogenesis attributed to multiple loci on chromosome 17. We have cloned the gene for an abundant testicular germ cell protein, t complex polypeptide 1, which has a variant form in t haplotypes, TCP-1A. A cDNA clone, pB1.4, which hybridizes to a 19S mRNA that is abundant in h...

journal_title：Cell

authors： Willison KR,Dudley K,Potter J

更新日期：1986-03-14 00:00:00

abstract：:A large (approximately 200 kd) subunit of cellular RNA polymerase II (Pol II) and a virus-encoded subunit of rabbit poxvirus (RPV) RNA polymerase (137 kd) react with common monoclonal antibodies. Hybridization studies with viral and cellular DNA clones confirm that the viral and cellular proteins are related. Followin...

journal_title：Cell

authors： Morrison DK,Moyer RW

更新日期：1986-02-28 00:00:00

abstract：:Nontransformed pre-B cells were induced to differentiate in vitro along several different but predictable pathways with only dendritic cells (DC) and concanavalin A-stimulated T lymphocytes. DC-T from spleen induced secretion of only IgM, whereas DC-T from Peyer's patches induced high levels of IgA and intermediate le...

journal_title：Cell

authors： Spalding DM,Griffin JA

更新日期：1986-02-14 00:00:00

abstract：:Conditions are described for programming with active transcription complexes essentially all the Xenopus 5S RNA genes added to an extract of Xenopus oocyte nuclei. We call this rate enhancement. Transcription by SP6 RNA polymerase of either strand of a Xenopus 5S RNA gene is unimpeded by the presence of a complete tra...

journal_title：Cell

authors： Wolffe AP,Jordan E,Brown DD

更新日期：1986-02-14 00:00:00

abstract：:Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion ...

journal_title：Cell

authors： Helmy S,Porter-Jordan K,Dawidowicz EA,Pilch P,Schwartz AL,Fine RE

更新日期：1986-02-14 00:00:00

abstract：:Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for...

journal_title：Cell

authors： Meeks-Wagner D,Wood JS,Garvik B,Hartwell LH

更新日期：1986-01-17 00:00:00

abstract：:To determine the function of c-fps in chicken macrophages and granulocytic cells we have infected chicken bone marrow cells with retroviruses containing the v-fps oncogene. Normal chicken macrophage progenitors, M-CFCs, give rise to macrophage colonies in semisolid cultures when macrophage colony stimulating factor (M...

journal_title：Cell

authors： Carmier JF,Samarut J

更新日期：1986-01-17 00:00:00

abstract：:A gel electrophoresis DNA binding assay has been used to identify proteins in HeLa cell extracts that specifically bind to the major late promoter of adenovirus. A major late promoter transcription factor MLTF has been detected as a discrete protein-DNA complex. MLTF binds specifically and with high affinity to sequen...

journal_title：Cell

authors： Carthew RW,Chodosh LA,Sharp PA

更新日期：1985-12-01 00:00:00

abstract：:The pathway of RNA polymerase entry at the lac promoter was studied by investigating the relationship between the promoter and a weak, overlapping polymerase interaction site (P2). If polymerase is made to enter the DNA by binding in vitro at this P2 site, cyclic AMP receptor protein (CRP) actively removes polymerase ...

journal_title：Cell

authors： Meiklejohn AL,Gralla JD

更新日期：1985-12-01 00:00:00

abstract：:We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...

journal_title：Cell

authors： de Ferra F,Engh H,Hudson L,Kamholz J,Puckett C,Molineaux S,Lazzarini RA

更新日期：1985-12-01 00:00:00

abstract：:Developmental analysis of a newly isolated maternal effect grandchildless mutant, tudor (tud), in Drosophila melanogaster indicates that tud+ activity is required during oogenesis for the determination and/or formation of primordial germ cells (pole cells) and for normal embryonic abdominal segmentation. Regardless of...

journal_title：Cell

authors： Boswell RE,Mahowald AP

更新日期：1985-11-01 00:00:00

abstract：:The replication initiator protein of the plasmid R6K binds to seven contiguous 22 bp direct repeats that form an indispensable part of the three replication origins alpha, beta, and gamma. Binding of the initiator to the direct repeats induced a marked bending of the region of gamma replication origin. Binding of the ...

journal_title：Cell

authors： Mukherjee S,Patel I,Bastia D

更新日期：1985-11-01 00:00:00